To achieve the objectives of the European Green Deal, the EU's Digital Agenda, and the Union of Equality, integrating an equality dimension into the work of the European Commission’s Directorate-General for Mobility and Transport (DG MOVE) is absolutely crucial. In its Sustainable and Smart Mobility Strategy, the European Commission commits to applying equality mainstreaming to its transport policy. To support the pursuit of this commitment, DG MOVE has developed a practical handbook to help staff apply equality mainstreaming in their daily work, and, hence, to comply with their obligations to promote equality and fight discrimination under Articles 8 and 10 of the Treaty on the Functioning of the European Union. This handbook can be useful for other organisations as well. The Equality Mainstreaming Handbook has been developed to serve as a reference document for self-learning or reviewing how to apply equality mainstreaming in daily processes. It provides concrete step-by-step guidance on how to put equality mainstreaming into practice and integrate it in all stages of the policy cycle with 10 practical and pragmatical tools, based on examples from the transport legislative framework. Equality mainstreaming is a systematic process of integrating an equality perspective into every stage of the policymaking cycle (i.e. preparation, implementation, monitoring and evaluation) and in all policy areas. It is relevant for all policy interventions and initiatives at all stages and for all staff. The rewards of a comprehensive equality mainstreaming approach to transport policies and other initiatives are multiple: increased attention to the safety concerns of disadvantaged groups of transport users, better provision of safe, affordable, and accessible transport options, increased uptake of sustainable transport options as well as a more diverse talent pool of transport workers.